FAMeS: Fidelity of Analysis of Metagenomic Samples

Assembly Method Comparison

The following graphs show the comparisons between the assembly methods.
You can view a list of contigs assembled for any combination of data set and assembly program.

Data set:


Assembler:

The following table contains a summary of the assembly methods used


simLC

simMC

simHC


Phrap

Arachne

JAZZ

Phrap

Arachne

JAZZ

Phrap

Arachne

JAZZ

Reads

100628

100628

100546

125652

125652

125513

118064

118064

117890

Contigs(>1 reads)

10320

1400

1953

12644

4692

5840

19236

558

1066

Homogeneous contigs

9055

1333

1941

11026

4329

5282

16451

477

1012

Reads in contigs

56843

33065

29390

82693

55022

39808

47338

2744

2832

Degree of assembly in contigs (%)

56.49%

32.86%

29.23%

65.81%

43.79%

31.71%

40.10%

2.32%

2.40%

Major contigs (≥10 reads)

482

367

503

1980

1372

876

86

20

11

Homogeneous contigs

287

330

502

1380

1133

605

5

18

9

Reads in major contigs

30852

28060

24405

50267

39190

19719

1521

290

192

Degree of assembly
in major contigs (%)

30.66%

27.88%

24.28%

40.00%

31.19%

15.71%

1.29%

0.25%

0.16%



The following graphs show the degree of chimerism for each of the contigs in a data set as assembled by a given program. For each data set, the first graph is coloured according to the assembly program used, the second according to the taxonomic level of chimerism.

Chimerism in the AMD sample by assembly program

Chimerism in the AMD sample by taxonomic level

Chimerism in the EBPR sample by assembly program

Chimerism in the EBPR sample by taxonomic level

Chimerism in the soil sample by assembly program

Chimerism in the soil sample by taxonomic level